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The use of microscopes and their components
The use of microscopes and their components
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1, Usemonocular tube biologic microscopetimeTo develop the habit of observing with the left eye (usually drawing with the right hand), both eyes should be opened at the same time during observation, and not one should be opened or closed, as this can easily lead to fatigue. To train students to observe with both eyes open at the same time, you can cut a piece about14cm， A rectangular piece of hard paper with a width of about 6cm, a circular hole with a diameter slightly smaller than the outer diameter of the upper end of the tube is dug near the left end, and the circular hole is placed on the upper part of the tube. When observing, both eyes are opened at the same time, and the right end of the paper is used to block the right eye's line of sight. After training for a period of time, you can get used to opening both eyes at the same time, and then remove the paper.
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1, Usemonocular tube biologic microscopetimeTo develop the habit of observing with the left eye (usually drawing with the right hand), both eyes should be opened at the same time during observation, and not one should be opened or closed, as this can easily lead to fatigue. To train students to observe with both eyes open at the same time, you can cut a piece about14cm， A rectangular piece of hard paper with a width of about 6cm, a circular hole with a diameter slightly smaller than the outer diameter of the upper end of the tube is dug near the left end, and the circular hole is placed on the upper part of the tube. When observing, both eyes are opened at the same time, and the right end of the paper is used to block the right eye's line of sight. After training for a period of time, you can get used to opening both eyes at the same time, and then remove the paper.
2、monocular tube biologic microscopeThe connection between the mirror arm and the mirror holder is a mechanical jointIt can be used to adjust the tilt of the lens barrel for easy observation, and the mirror arm should not tilt too far back, generally not exceeding 40 °. However, when using temporary mounting for observation, it is prohibited to use tilt joints (when the lens barrel tilts, the stage also tilts, and the liquid on the glass slide is prone to flow out), especially when the mounting contains acidic reagents, to avoid staining the mirror body.
3. The use of eyepiece and objective lens
Generallymonocular tube biologic microscopeWe start observing with a moderate magnification eyepiece (10x) and a low magnification objective, gradually switching to a higher magnification objective to find the magnification that meets the experimental requirements.
When changing the objective lens, first observe with a low magnification lens and adjust to the correct working distance (clear imaging). If further using a high-power objective for observation, the part of the object image that needs to be magnified for observation should be moved to the center of the field of view before converting to a high-power objective (when converting a low-power objective to a high-power objective for observation, the range of the object image in the field of view is greatly reduced). The low-power objective lens and high-power objective lens are basically in focus (same high focus). When observing clearly with a low-power objective lens, changing to a high-power objective lens should allow the object image to be seen, but the object image may not be very clear. The fine focus screw can be rotated to adjust it.
It is generally believed that usingmonocular tube biologic microscopeWhen using any objective lensThe upper limit of effective magnification is 1,000Multiplying by its numerical aperture, the lower limit is250Multiply its numerical aperture. in compliance with40What is the numerical aperture of the objective lens0.65The upper and lower limits are:1000×0.65=650Beihe and250×0.65≈163Exceeding the upper limit of effective magnification is called ineffective magnification, which cannot improve the observation effect.Magnification below the lower limit is difficult for the human eye to distinguish and is not conducive to observation. The general practical magnification range is500- A number between 700 times the numerical aperture.
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